{"product_id":"formedium-m9-minimal-salts-base-5x","title":"M9 Minimal Salts Base, 5x","description":"\u003c!----\u003e\u003cdiv class=\"beae-des-content\"\u003e\n\u003cp\u003eM9 Minimal Salts Base, 5x is used in preparing M9 Minimal medium used for the cultivation and maintenance of E. coli in molecular biology.\u003c\/p\u003e\n\u003cp\u003eM9 is a chemical defined minimal medium supporting the growth of \"wild-type\" strains of E.coli. The medium can be supplemented with specific amino acids or other required nutrients allowing for selection of specific auxotrophs.\u003c\/p\u003e\n\u003cp\u003eGlucose may be added as a source of carbohydrate. Nitrogen is provided by ammonium chloride final osmolarity of the medium is maintained by NaCl. Addition of magnesium and calcium increases the growth of the recombinant cells.\u003c\/p\u003e\n\u003cp\u003eM9 Minimal Salts Base, 5x is a 5x concentrate and has to be diluted to a 1x concentration and subsequently supplemented with an appropriate carbon source, such as glucose. Addition of required nutritionally elements may be added.\u003c\/p\u003e\n\u003cp\u003eDifco Manual, 11th edition, Sparks, MD, 272, 1998. Davis, L.G., M.D. Dibner and J.F. Battey, Basic methods in molecular biology, Elsevier, new York, (1986). Sambrook, J,., E. F. Fritsch, and T. Maniatis, 1989, Molecular cloning: a laboratory manual, 2nd edition ed., Cold Spring Harbour laboratory, Cold Spring Harbour, N.Y.\u003c\/p\u003e\n\u003ctable style=\"font-family: Saira; border-collapse: collapse; width: 100%;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align: left; border: 1px solid #000;\"\u003e\n\u003ch2\u003eFormula\u003c\/h2\u003e\n\u003c\/th\u003e\n\u003cth style=\"text-align: left; border: 1px solid #000;\"\u003e\n\u003ch2\u003eg\/l\u003c\/h2\u003e\n\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #000;\"\u003eNa2HPO4\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #000;\"\u003e33.9\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #000;\"\u003eKH2PO4\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #000;\"\u003e15\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #000;\"\u003eNaCl\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #000;\"\u003e2.5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #000;\"\u003eNH4Cl\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #000;\"\u003e5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #000;\"\u003eFinal pH\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #000;\"\u003e6.8 ± 0.2 at 25°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003eSuspend 56.4 gram powdered medium in 1 litre distilled water\u003c\/p\u003e\n\u003cp\u003eStore dry at room temperature.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Formedium","offers":[{"title":"6 x 1kg","offer_id":51824666509348,"sku":"MMS0103","price":1198.14,"currency_code":"USD","in_stock":true},{"title":"1000g","offer_id":51824666542116,"sku":"MMS0102","price":223.63,"currency_code":"USD","in_stock":true},{"title":"250g","offer_id":51824666574884,"sku":"MMS0101","price":69.46,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0589\/9868\/5732\/files\/formedium-m9-minimal-salts-base-5x-1_f22e2e1c-dff3-49e1-a2ec-ef4c25dd055f.jpg?v=1790072182","url":"https:\/\/vareum.com\/products\/formedium-m9-minimal-salts-base-5x","provider":"Vareum","version":"1.0","type":"link"}