Rat uPA(Plasminogen Activator, Urokinase) ELISA Kit

Item number: ELK10436
Availability: In Stock
Product Type: Precoated
$458.00
$458.00
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Size: 96T
Subtotal: $458.00
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Rat uPA(Plasminogen Activator, Urokinase) ELISA Kit

Rat uPA(Plasminogen Activator, Urokinase) ELISA Kit

$458.00

Rat uPA(Plasminogen Activator, Urokinase) ELISA Kit

$458.00
Size: 96T
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat uPA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat uPA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat uPA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat uPA in the samples is then determined by comparing the OD of the samples to the standard curve.
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Research area
Tumor immunity;
Assay length
3.5h
Sample type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Detection range
0.16-10 ng/mL
Standard
10 ng/mL
Sensitivity
0.061 ng/mL
Assay type
Sandwich
Reactivity
Rat

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