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Home Collection Biochemical Assay

Biochemical Assay

Collection: Biochemical Assay

High-performance assays for the detection, quantification, and analysis of biological molecules.

Proteins

Proteins

Biochemical Assay

Biochemical Assay

ELISA Kit

ELISA Kit

Gel Electrophoresis

Gel Electrophoresis

Molecular

Molecular

Antibodies

Antibodies

Cell Culture

Cell Culture

Lab Consumables

Lab Consumables

Laboratory Equipment

Laboratory Equipment

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Abmole 505
Advanced BioMatrix 4
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Applications

600μL (to be able to compare the results, the sample was diluted 1:2 with Binding buffer and filtered through 0.2μm). 1
Alkali tolerance, high capacity and low ligand leakage in combination with the rigid base matrix makes MabSelect SuRe LX an excellent choice for purification of monoclonal antibodies for clinical applications. 1
Applications include the production of large quantities of cells, viruses and recombinant cell products (e.g. interferon, enzymes, nucleic acids, hormones), studies on cell adhesion, diff erentiation and cell function, perfusion column culture systems, microscopy studies, harvesting mitotic cells, i... 3
Can be operated with a syringe, peristaltic pump or liquid chromatography system such as ÄKTA design. 1
Capto L is suitable for capture of a wide range of antibody fragments such as Fabs, single-chain variable fragments (scFv), and domain antibodies (Dabs). 1
CBMC-MCpore2 macroporous microcarriers are principally designed for use in suspension culture systems for growth of adherent diploid cells requiering a higher surface charge, and the production of recombinant proteins. 1
CBMC-MCpore microcarriers are designed for use in stirred suspension culture systems for the growth of adherent recombinant CHO cells and the production of recombinant proteins. 1
Centrifugation ; Vacuum 10
Columns can be used with a standard microcentrifuge and one purification run takes less than 20 min Designed for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants. 1
Contaminant removal to formulation levels in post protein A purification. 1
Designed for small-scale sample preparation for single use: Enrichment of target protein Protein-protein interaction studies Upstream of gel electrophoresis, liquid chromatography and mass spectrometry. 1
Designed for use with a syringe, peristaltic pump, or chromatography system. 1
Designed for use with syringe, peristaltic pump and other chromatography systems. 2
Designed for use with syringe, peristaltic pump, and other chromatography systems. 1
Enrichment of target proteins Purification of antibodies. 1
Fast and efficient manual purification of monoclonal and polyclonal antibodies. 3
For fast and simple manual purification of GST-tagged proteins by gravity flow. 1
For fractionation of lipids, hormones, vitamins, and other small biomolecules in polar organic solvents and aqueous solvent mixtures. 1
For manufacturing of biopharmaceuticals for clinical phase I and II studies. Depending on the scale of operations, the columns can also be used for full-scale manufacturing, as well as for preclinical studies. 1
For manufacturing of biopharmaceuticals for clinical phase I and II studies. Depending on the scale of operations, they can also be used for full-scale manufacturing, as well as for preclinical studies. 19
For manufacturing of biopharmaceuticals for clinical phase I, II, and III studies. Depending on the scale of operations, they can also be used for full-scale manufacturing, as well as for preclinical studies. 2
For method scouting, group separations, sample concentration and sample clean-up of charged biomolecules 1
For method scouting, group separations, sample concentration and sample clean-up of charged biomolecules. 4
For purification of GST-tagged proteins. 1
For purification, isolation or removal of molecules containing branched mannoses with fucose linked α(1,6) to the N-acetylglucosamine, (αMan>αGlc>GlcNAc) and sterically related residues like glycoproteins, membrane proteins, glycolipids, lipoproteins, pol. 1
For research use only. 1
For Research Use Only. Not intended for any clinical use. 178
For screening of selectivity, binding and elution conditions, as well as small scale purifications. 7
For simple, small-scale purification of GST-tagged proteins and rapid expression screening by centrifugation. 1
For the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins and for preparation of cytoplasmic extract. 1
For the removal and/or purification of serine proteases e.g. thrombin. 1
For the separation of molecules Mr >30 000 from molecules Mr <1500 such as labeled protein or DNA from unconjugated dyes For removeing small nucleotides from longer chain nucleic acids. 2
For using Capto Heparin to purify the blood coagulation factor antithrombin III from bovine plasma. 1
GammaBind Plus Sepharose is used to analyze and purify classes, subclasses, and fragments of immunoglobulins from any biological fluid or cell culture medium. This resin is also suitable for immunoprecipitation. 1
Glutathione S-transferase (GST) tagged proteins produced using the pGEX series of expression vectors, Other glutathione S-transferases and glutathione-dependent proteins. 1
GSTrap 4B columns are used for the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function. 1
Heparin resin allows for fast and reliable separations of biomolecules with an affinity for heparin, including antithrombin III antibodies, coagulation factors, and other plasma proteins. DNA-binding proteins, lipoproteins, protein synthesis factors, enzymes that act on nucleic acids, and steroid re... 1
His Buffer Kit can be used in combination with a number of column types, such as His SpinTrap, His MultiTrap FF, His MultiTrap HP, His GraviTrap, HisTrap FF, HisTrap FF crude and HisTrap HP. 1
Intended for research use only, and shall not be used in any clinical or in vitro procedures for diagnostic purposes. 1
It is suitable for the isolation of exosomes from the supernatant of a variety of cells. And the exosomes isolated by this kit are comparable to ultracentrifugation with High Purity and High Yield. Moreover, this kit is simple to use and no equipment requirement. 1
KappaSelect is an affinity chromatography resin designed for the purification of Fab (kappa) with high binding capacity, purity and yield. 1
LambdaFabSelect is an affinity resin designed for the purification of human lambda Fab fragments. The ligand binds to the constant region of the light chain enabling an efficient capture step for human antibody fragments such as Fabs with high purity and yield. 1
Microcarrier-based culturing can be performed in simple shaker flasks or roller bottles as well as in more advanced traditional or single-use bioreactor systems, from milliliter to thousands-of-liter scales. Compared with cultivation in several shake flasks or roller bottles, one single bioreactor c... 1
Purification is done under physiological conditions and mild elution preserves the activity of the target protein. 1
Purification of antibodies such as human IgG 2
Purification of antibodies such as human IgG. 2
Purification of his-tagged proteins 1
Purification of most untagged or native proteins. 1
Purification of recombinant proteins tagged with Strep-tag II and Twin-Strep-tag. 2
purifying Factor VII spiked in human plasma. 1
Recombinant tagged proteins containing a protein A tag. 1
Sample volume of up to 150 μl undiluted human plasma results in more than 95% albumin depletion and more than 90% IgG depletion. 1
The interaction between streptavidin and biotin is very strong and requires denaturing conditions for elution. This strong interaction can be utilized in the purification of antigens. Biotinylated antibody-antigen complexes bind to the HiTrap Streptavidin HP column, enabling subsequent elution of th... 1
The MabSelect family, comprising MabSelect, MabSelect Xtra, MabSelect SuRe, and MabSelect SuRe LX chromatography media, was developed for high-capacity MAb capture at process scale. To enable the high mass transfer required for continuous chromatography, MabSelect SuRe pcc was further developed from... 1
The most important application areas for Blue Sepharose 6 Fast Flow are the purification of interferon and albumin, as well as albumin removal. The high capacity and high flow rates make the resin suitable for both laboratory and process scale separations 1
The resin has a long hydrophilic spacer arm which makes it particular suitable for immobilization of small molecules. 1
Used for protein separation. 10
Used for purification of IgM. 1
Used for purification of IgY from egg yolk The lipids from an egg yolk were removed by precipitation with water and centrifugation at 4°C. 1
Used for site-specific separation of the GST tag from proteins expressed using pGEX-6P vectors 1
Used for site-specific separation of the GST tag from proteins expressed using pGEX-T vectors. 1
Western Blots 1
When using AVB Sepharose High Performance the AAV can be applied directly from clarified AAV vector cell lysate. Conventional buffers (e.g., PBS, Tris, citrate) may be used for loading, washing, and elution. Virus binds to the column at around neutral pH and is typically eluted by lowering the pH, f... 1

Storage Temp

-20°C 2
2-4°C 1
4°C 4
2–8°C 3
4°C, Protect from light. 12
4ºC 10
4ºC Do not freeze 9
4ºC. Do not freeze. 1
-20 ℃ 23
2-8 ℃ 11
2 - 8°C, storage buffer: 20% ethanol 2
2 - 8°C, storage buffer: 20% ethanol as preservative 1
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C. 1
at -20°C 1
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months. 2
Desiccate at 4° C. Store solution in aliquots at -20°C. Protect from light. 3
Multiple Temperatures 9
Preservation temperature 2~8℃ for long-term storage, 2 years 1
RT 15
RT and 4ºC 1
RT or 4ºC 1
Stable for at least 1 year after receipt when stored at -20°C. 1
Stable for at least 1 year after receipt when stored at +4°C. 2
Stable for at least 2 years after receipt when stored at -20°C. 13
Stable for at least 2 years after receipt when stored at +20°C. 1
Stable for at least 2 years after receipt when stored at +4°C. 30
Stable for at least 3 years after receipt when stored at -20°C. 2
Stable storage at 2 ~ 8°C, shelf life of 2 years (can be stored or transported for a short time at room temperature). 57
Store at 2ºC to 8ºC. Do not freeze. 1
Store at 4° C 1
Store at 4℃ or lower. Protect from light. 4
Store at 4℃ or lower. Protect from light. Other properties are not known. 1
Store at -20° C 1
Store at -20°C 1
Store protected from light at 2−8 °C. Do not freeze. The expiration date is indicated on the vial label. 1
Store unused media in its container at a temperature of 2 to 8°C for long term storage. 491
Stored at -20°C. Avoid repeated freeze-thaw cycles. 4
The kit should be stored dry at room temperature for up to one month. For longer storage, the kit can be kept at 2-8ºC for up to 24 months. Do not freeze the kit or any of its components. 2
The Rapid Endotoxin Detection Kit is stable at room temperature and does not require refrigeration for shipping or storage. 1
Unopened kits are stable at 2–8°C until the expiry date printed on the label. 3

Type

Biochemical Assay 1313
Molecular 1

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