Recombinant E.coli Trp synthase (N-6His)

Item number: EPT234
Availability: In Stock
$589.00
$589.00
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Size: 50ug
Subtotal: $589.00
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Recombinant E.coli Trp synthase (N-6His)

Recombinant E.coli Trp synthase (N-6His)

$589.00

Recombinant E.coli Trp synthase (N-6His)

$589.00
Size: 50ug
Description
Recombinant E.coli Tryptophan Synthase is produced by our E.coli expression system and the target gene encoding Met1-Ser268&Thr2-Ile397 is expressed with a 6His tag at the N-terminus.
Accession
P0A877&P0A879
Molecular weight
28.7&43.8 KDa
Apparent molecular weight
28&40-50 KDa, reducing conditions
Other names
Tryptophan synthetase; Tryptophan synthase
Purity
Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin
Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Storage
Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.
Delivery condition
The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.
Tryptophan synthase is a multienzyme α2β2 complex composed of two protein subunit. Tryptophan synthase catalyzes the last two steps in the synthesis of L-tryptophan (L-Trp). The α-subunit catalyzes cleavage of 3-indole-d-glycerol 3′-phosphate (IGP) to give indole and D-glyceraldehyde 3′-phosphate (G3P). Indole is then transferred through a 25-Å hydrophobic tunnel to the β-subunit. The β2 subunit contains pyridoxal 5'-phosphate and catalyzes several pyridoxal 5'-phosphate-dependent reactions, including/3-elimination reactions 6 and a thiol-dependent transamination reaction. This enzyme is commonly found in Eubacteria, Archaebacteria, Protista, Fungi, and Plantae, but is absent from Animalia. As humans do not have tryptophan synthase, this enzyme has been explored as a potential drug target.

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