Recombinant Human IL-2 (aldesleukin)

Item number: EPT147
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$99.00
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Recombinant Human IL-2 (aldesleukin)

Recombinant Human IL-2 (aldesleukin)

$99.00

Recombinant Human IL-2 (aldesleukin)

$99.00
Size: 10ug
Description
Recombinant Human Interleukin-2 is produced by our E.coli expression system and the target gene encoding Pro22-Thr153(Cys145Ser) is expressed.
Accession
P60568
Molecular weight
15.5 KDa
Apparent molecular weight
14 KDa, reducing conditions
Other names
Interleukin-2; IL-2; T-cell growth factor; TCGF; Aldesleukin
Purity
Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin
Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Biological activity
Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. The ED50 for this effect is 90-270 pg/ml.
Redissolve
Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Delivery condition
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Recombinant Human Interleukin-2 is a highly purified protein with a molecular weight of approximately 15,300 Daltons. The chemical name is des-alanyl-1, serine-145 Human Interleukin-2. It is produced by recombinant DNA technology using a genetically engineered E. coli strain containing an analog of the human interleukin-2 gene. Genetic engineering techniques were used to modify the Human IL-2 gene, and the resulting expression clone encodes a modified Human IL-2. This recombinant form differs from native Interleukin-2 in following ways: it is not glycosylated; the molecule has serine substituted for cysteine at amino acid position 145; the aggregation state of molecule is likely to be different from that of native IL-2.

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