Recombinant Human/Murine/Rat BDNF

Item number: EPT036
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$819.00
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Recombinant Human/Murine/Rat BDNF

Recombinant Human/Murine/Rat BDNF

$819.00

Recombinant Human/Murine/Rat BDNF

$819.00
Size: 50ug
Description
Recombinant Human Brain-Derived Neurotrophic Factor is produced by our E.coli expression system and the target gene encoding His129-Arg247 is expressed.
Accession
P23560
Molecular weight
13 KDa
Apparent molecular weight
14 KDa, reducing conditions
Other names
Brain-Derived Neurotrophic Factor; BDNF; Abrineurin
Purity
Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin
Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test.
Biological activity
Immobilized Human TrkB-His (Cat#C507) at 2ug/ml (100 μl/well) can bind Human BDNF*(Cat#C076)*: Biotinylated by NHS-biotin prior to testing. The ED50 of Human BDNF*(Cat#C076) is 47.58 ng/ml.
Redissolve
Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Delivery condition
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Brain-Derived Neurotrophic Factor (BDNF) is a member of the neurotrophin family. Along with other structurally related neurotrophic factors NGF, NT-3 and NT-4, BDNF binds with high affinity to the TrkB kinase receptor. It also binds with the LNGFR (for low-affinity nerve growth factor receptor, also known as p75). BDNF promotes the survival, growth and differentiation of neurons. It serves as a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. BDNF expression is altered in neurodegenerative disorders such as Parkinson's and Alzheimer's disease.

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