Recombinant Human NEDD8 (N-6His-SUMO)

Item number: EPT278
Availability: In Stock
$39.00
$39.00
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Size: 10ug
Subtotal: $39.00
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Recombinant Human NEDD8 (N-6His-SUMO)

Recombinant Human NEDD8 (N-6His-SUMO)

$39.00

Recombinant Human NEDD8 (N-6His-SUMO)

$39.00
Size: 10ug
Description
Recombinant Human Neural Precursor Cell Expressed Developmentally Down-regulated Protein 8 is produced by our E.coli expression system and the target gene encoding Met1-Gly76 is expressed with a 6His, SUMO tag at the N-terminus.
Accession
Q15843
Molecular weight
20.9 KDa
Apparent molecular weight
24 KDa, reducing conditions
Other names
Neural precursor cell expressed developmentally down-regulated protein 8; NEDD8; Neddylin; Ubiquitin-like protein Nedd8
Purity
Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin
Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Redissolve
Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Delivery condition
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Human NEDD8 shares 60% amino acid sequence identity to ubiquitin. The only known substrates of NEDD8 modification are the cullin subunits of SCF ubiquitin E3 ligases. The NEDDylation of cullins is critical for the recruitment of E2 to the ligase complex, thus facilitating ubiquitin conjugation. NEDD8 modification has therefore been implicated in cell cycle progression and cytoskeletal regulation.

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