Recombinant Human NovoNectin

Item number: EPT094
Availability: In Stock
$149.00
$149.00
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Size: 50ug
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Recombinant Human NovoNectin

Recombinant Human NovoNectin

$149.00

Recombinant Human NovoNectin

$149.00
Size: 50ug
Description
Recombinant Human Fibronectin Fragment is produced by our E.coli expression system and the target gene encoding Pro1270-Ser1546&Ala1721-Thr2016 is expressed.
Accession
P02751
Molecular weight
62.7 KDa
Apparent molecular weight
60-80 KDa, reducing conditions
Other names
NovoNectin; Fibronectin; FN; Cold-insoluble globulin; CIG; FN; Fibronectin 1
Purity
Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin
Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test.
Biological activity
Measured by its ability to support cell attachment and spreading when used as a substratum for cell culture. The ED50 for this effect is 0.1-0.5 ug/ml.
Redissolve
Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Delivery condition
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Fibronectin1(FN1) is a secreted protein and contains 12 fibronectin type-I domains,fibronectin type-II domains and 16 fibronectin type-III domains.Recombinant human fibronectin fragment, is a protein of ~63 kDa containing a central cell-binding domain, a high affinity heparin-binding domain II,and CS1 site within the alternatively spliced III CS region of human fibronectin. Cells bind to a VLA-4 ligand, a CS-I site, and a VLA-5 ligand, a cell attachment domain, and virus vectors binds to a heparin binding domain II, which co-locates the cell and the virus vector on NovoNectin. This process enhances the density of both cells and vectors, and facilitates the gene transduction in the result.

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