Recombinant Rat TNF alpha

Item number: EPT089
Availability: In Stock
$589.00
$589.00
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Size: 50ug
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Recombinant Rat TNF alpha

Recombinant Rat TNF alpha

$589.00

Recombinant Rat TNF alpha

$589.00
Size: 50ug
Description
Recombinant Rat Tumor Necrosis Factor is produced by our E.coli expression system and the target gene encoding Leu80-Leu235 is expressed.
Accession
P16599
Molecular weight
17.4 KDa
Apparent molecular weight
14 KDa, reducing conditions
Other names
Tumor Necrosis Factor; Cachectin; TNF-Alpha; Tumor Necrosis Factor Ligand Superfamily Member 2; TNF-a; Tumor Necrosis Factor; Membrane Form; Tumor Necrosis Factor; Soluble Form; Tnf; Tnfa; Tnfsf2
Purity
Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin
Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Redissolve
Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Delivery condition
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Tumor necrosis factor alpha (TNF-alpha, TNFSF2) is the prototypic ligand of the TNF superfamily. Rat TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 179 aa extracellular domain (ECD). Within the ECD, rat TNF-alpha shares 94% aa sequence identity with mouse. TNF-alpha is produced by a wide variety of immune, epithelial, endothelial, and tumor cells. TNF exists as a homotrimer and interacts with SPPL2B. TNF is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. TNF is a key cytokine in the development of several inflammatory disorders. It contributes to the development of type 2 diabetes throught its effects on insulin resistance and fatty acid metabolism.

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