Product Overview
Product Overview
Assay length
3.5h
Assay type
Sandwich
Detection range
0.16-10 ng/mL
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Reactivity
Human
Research area
Signal transduction;CD & Adhesion molecule;Immune molecule;
Sample type
tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
0.067 ng/mL
Standard
10 ng/mL
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CD4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CD4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CD4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CD4 in the samples is then determined by comparing the OD of the samples to the standard curve.
Uniprot id
P01730
Background
Background
Product Citations
Product Citations
More Info
More Info
Human CD4(Cluster Of Differentiation 4) ELISA Kit
Human CD4(Cluster Of Differentiation 4) ELISA Kit
Regular price
$458.00 USD
Sale price
$458.00 USD
Regular price
Unit price
/per
Shipping calculated at checkout.
Available in stock (100)
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For research use only — not intended for clinical or diagnostic purposes.
Product Overview
Product Overview
Assay length
3.5h
Assay type
Sandwich
Detection range
0.16-10 ng/mL
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Reactivity
Human
Research area
Signal transduction;CD & Adhesion molecule;Immune molecule;
Sample type
tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
0.067 ng/mL
Standard
10 ng/mL
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CD4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CD4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CD4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CD4 in the samples is then determined by comparing the OD of the samples to the standard curve.
Uniprot id
P01730
Background
Background
Product Citations
Product Citations
More Info
More Info
Human CD4(Cluster Of Differentiation 4) ELISA Kit
Regular price
$458.00 USD
Sale price
$458.00 USD
Regular price
Unit price
/per
