Mouse NFκB-p65(Nuclear Factor Kappa B p65) ELISA Kit

Item number: ELK8449
Availability: In Stock
Product Type: Precoated
$320.00
$320.00
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Size: 48T
Subtotal: $320.00
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Mouse NFκB-p65(Nuclear Factor Kappa B p65) ELISA Kit

Mouse NFκB-p65(Nuclear Factor Kappa B p65) ELISA Kit

$320.00

Mouse NFκB-p65(Nuclear Factor Kappa B p65) ELISA Kit

$320.00
Size: 48T
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NFκB-p65. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NFκB-p65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NFκB-p65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NFκB-p65 in the samples is then determined by comparing the OD of the samples to the standard curve.
Uniprot id
Q04207
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Research area
Apoptosis;
Assay length
3.5h
Sample type
Tissue homogenates, cell lysates and other biological fluids
Detection range
31.25-2000 pg/mL
Standard
2000 pg/mL
Sensitivity
11.5 pg/mL
Assay type
Sandwich
Reactivity
Mouse

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