BH2(Dihydrobiopterin) ELISA Kit

Item number: ELK9938
Availability: In Stock
Product Type: Precoated
$458.00
$458.00
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Size: 96T
Subtotal: $458.00
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BH2(Dihydrobiopterin) ELISA Kit

BH2(Dihydrobiopterin) ELISA Kit

$458.00

BH2(Dihydrobiopterin) ELISA Kit

$458.00
Size: 96T
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with BH2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to BH2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of BH2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Research area
Signal transduction
Assay length
3.5h
Sample type
serum, plasma, cell lysates, cell culture supernates and other biological fluids
Detection range
31.25-2000 pg/mL
Standard
2000 pg/mL
Sensitivity
11.31 pg/mL
Assay type
Competitive Inhibition
Reactivity
General

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