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Caffeine ELISA Kit
Caffeine ELISA Kit

Caffeine ELISA Kit

Caffeine ELISA Kit

Vendor: ELK Biotechnology

Type: Precoated

SKU: ELK0683

$458.00
$458.00
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Documents

Manual
SDS

Available in stock (100)

Size: 96T
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Product Overview

Assay length
2.5h
Assay type
Competitive Inhibition
Detection range
0.47-30 ng/mL
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Reactivity
General
Research area
Signal transduction;
Sample type
Serum, plasma, other biological fluids
Sensitivity
0.25 ng/mL
Standard
30 ng/mL
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Caffeine. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Caffeine. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Caffeine in the samples is then determined by comparing the OD of the samples to the standard curve.
Caffeine ELISA Kit

Caffeine ELISA Kit

$458.00
$458.00