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cAMP(Cyclic Adenosine Monophosphate) ELISA Kit
cAMP(Cyclic Adenosine Monophosphate) ELISA Kit

cAMP(Cyclic Adenosine Monophosphate) ELISA Kit

cAMP(Cyclic Adenosine Monophosphate) ELISA Kit

Vendor: ELK Biotechnology

Type: Precoated

SKU: ELK8116

$370.00
$370.00
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Manual
SDS

Available in stock (100)

Size: 48T
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Product Overview

Assay length
2h
Assay type
Competitive Inhibition
Detection range
312.5-20000 pg/mL
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Reactivity
General
Research area
Signal transduction;Metabolic pathway;
Sample type
serum, plasma, cell lysates, cell culture supernates and other biological fluids
Sensitivity
92.2 pg/mL
Standard
20000 pg/mL
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with cAMP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to cAMP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of cAMP in the samples is then determined by comparing the OD of the samples to the standard curve.
cAMP(Cyclic Adenosine Monophosphate) ELISA Kit

cAMP(Cyclic Adenosine Monophosphate) ELISA Kit

$370.00
$370.00