Cattle CA1(Carbonic Anhydrase I) ELISA Kit

Item number: ELK10892
Availability: In Stock
Product Type: Precoated
$458.00
$458.00
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Size: 96T
Subtotal: $458.00
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Cattle CA1(Carbonic Anhydrase I) ELISA Kit

Cattle CA1(Carbonic Anhydrase I) ELISA Kit

$458.00

Cattle CA1(Carbonic Anhydrase I) ELISA Kit

$458.00
Size: 96T
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle CA1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle CA1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle CA1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle CA1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Uniprot id
Q1LZA1
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Research area
Enzyme & Kinase;Bone metabolism;
Assay length
3.5h
Sample type
Serum, plasma, tissue homogenates, cell lysates and other biological fluids
Detection range
0.16-10 ng/mL
Standard
10 ng/mL
Sensitivity
0.053 ng/mL
Assay type
Sandwich
Reactivity
Cattle

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