Cattle CD40L(Cluster of Differentiation 40 Ligand) ELISA Kit

Item number: ELK9956
Availability: In Stock
Product Type: Precoated
$458.00
$458.00
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Size: 96T
Subtotal: $458.00
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Cattle CD40L(Cluster of Differentiation 40 Ligand) ELISA Kit

Cattle CD40L(Cluster of Differentiation 40 Ligand) ELISA Kit

$458.00

Cattle CD40L(Cluster of Differentiation 40 Ligand) ELISA Kit

$458.00
Size: 96T
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle CD40L. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle CD40L. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle CD40L, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle CD40L in the samples is then determined by comparing the OD of the samples to the standard curve.
Uniprot id
P51749
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Research area
Signal transduction;CD & Adhesion molecule;Apoptosis;Tumor immunity;Infection immunity;Immune molecule;
Assay length
3.5h
Sample type
serum, plasma, tissue homogenates, cell lysates and other biological fluids
Detection range
0.63-40 ng/mL
Standard
40 ng/mL
Sensitivity
0.221 ng/mL
Assay type
Sandwich
Reactivity
Cattle

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