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Cattle ECF(Eosinophil Chemotactic Factor) ELISA Kit
Cattle ECF(Eosinophil Chemotactic Factor) ELISA Kit

Cattle ECF(Eosinophil Chemotactic Factor) ELISA Kit

Cattle ECF(Eosinophil Chemotactic Factor) ELISA Kit

Vendor: ELK Biotechnology

Type: Precoated

SKU: ELK5968

$412.00
$412.00
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Manual
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Available in stock (100)

Size: 48T
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Product Overview

Assay length
3.5h
Assay type
Sandwich
Detection range
62.5-4000 pg/mL
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Reactivity
Cattle
Research area
Cytokine;Infection immunity;Autoimmunity;
Sample type
Serum, plasma and other biological fluids.
Sensitivity
21 pg/mL
Standard
4000 pg/mL
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle ECF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle ECF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle ECF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle ECF in the samples is then determined by comparing the OD of the samples to the standard curve.
Cattle ECF(Eosinophil Chemotactic Factor) ELISA Kit

Cattle ECF(Eosinophil Chemotactic Factor) ELISA Kit

$412.00
$412.00