Cattle HIF1a(Hypoxia Inducible Factor 1 Alpha) ELISA Kit

Item number: ELK0283
Availability: In Stock
Product Type: Precoated
$458.00
$458.00
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Size: 96T
Subtotal: $458.00
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Cattle HIF1a(Hypoxia Inducible Factor 1 Alpha) ELISA Kit

Cattle HIF1a(Hypoxia Inducible Factor 1 Alpha) ELISA Kit

$458.00

Cattle HIF1a(Hypoxia Inducible Factor 1 Alpha) ELISA Kit

$458.00
Size: 96T
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle HIF1a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle HIF1a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle HIF1a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle HIF1a in the samples is then determined by comparing the OD of the samples to the standard curve.
Uniprot id
Q9XTA5
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Research area
Tumor immunity;Cardiovascular biology;Reproductive science;
Assay length
3.5h
Sample type
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection range
78.13-5000 pg/mL
Standard
5000 pg/mL
Sensitivity
27 pg/mL
Assay type
Sandwich
Reactivity
Cattle

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