Cattle PGFM(Prostaglandin F Metabolite) ELISA Kit

Item number: ELK0504
Availability: In Stock
Product Type: Precoated
$458.00
$458.00
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Size: 96T
Subtotal: $458.00
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Cattle PGFM(Prostaglandin F Metabolite) ELISA Kit

Cattle PGFM(Prostaglandin F Metabolite) ELISA Kit

$458.00

Cattle PGFM(Prostaglandin F Metabolite) ELISA Kit

$458.00
Size: 96T
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle PGFM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle PGFM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle PGFM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle PGFM in the samples is then determined by comparing the OD of the samples to the standard curve.
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Research area
Signal transduction;
Assay length
3.5h
Sample type
serum, plasma, tissue homogenates and other biological fluids
Detection range
15.63-1000 pg/mL
Standard
1000 pg/mL
Sensitivity
6.91 pg/mL
Assay type
Sandwich
Reactivity
Cattle

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