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Cattle S100(S100 Calcium Binding Protein) ELISA Kit
Cattle S100(S100 Calcium Binding Protein) ELISA Kit

Cattle S100(S100 Calcium Binding Protein) ELISA Kit

Cattle S100(S100 Calcium Binding Protein) ELISA Kit

Vendor: ELK Biotechnology

Type: Precoated

SKU: ELK5966

$412.00
$412.00
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Documents

Manual
SDS

Available in stock (100)

Size: 48T
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Product Overview

Assay length
3.5h
Assay type
Sandwich
Detection range
78.13-5000 pg/mL
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Reactivity
Cattle
Research area
Tumor immunity;
Sample type
Serum, plasma, tissue homogenates and other biological fluids.
Sensitivity
31 pg/mL
Standard
5000 pg/mL
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle S100. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle S100. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle S100, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle S100 in the samples is then determined by comparing the OD of the samples to the standard curve.
Cattle S100(S100 Calcium Binding Protein) ELISA Kit

Cattle S100(S100 Calcium Binding Protein) ELISA Kit

$412.00
$412.00