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Cattle VF(Visfatin) ELISA Kit
Cattle VF(Visfatin) ELISA Kit

Cattle VF(Visfatin) ELISA Kit

Cattle VF(Visfatin) ELISA Kit

Vendor: ELK Biotechnology

Type: Precoated

SKU: ELK5894

$412.00
$412.00
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Manual
SDS

Available in stock (100)

Size: 48T
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Product Overview

Assay length
3.5h
Assay type
Sandwich
Detection range
3.13-200 ng/mL
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Reactivity
Cattle
Research area
Metabolic pathway;Cardiovascular biology;Hepatology;
Sample type
Serum, plasma and other biological fluids.
Sensitivity
1.31 ng/mL
Standard
200 ng/mL
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle VF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle VF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle VF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle VF in the samples is then determined by comparing the OD of the samples to the standard curve.
Cattle VF(Visfatin) ELISA Kit

Cattle VF(Visfatin) ELISA Kit

$412.00
$412.00