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Chicken BALP(Bone-specific Alkaline Phosphatase) ELISA Kit
Chicken BALP(Bone-specific Alkaline Phosphatase) ELISA Kit

Chicken BALP(Bone-specific Alkaline Phosphatase) ELISA Kit

Chicken BALP(Bone-specific Alkaline Phosphatase) ELISA Kit

Vendor: ELK Biotechnology

Type: Precoated

SKU: ELK9183

$320.00
$320.00
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Manual
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Available in stock (100)

Size: 48T
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Product Overview

Assay length
3.5h
Assay type
Sandwich
Detection range
0.16-10 ng/mL
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Reactivity
Chicken
Research area
Enzyme & Kinase;Metabolic pathway;Hepatology;Bone metabolism;
Sample type
serum, plasma and other biological fluids
Sensitivity
0.061 ng/mL
Standard
10 ng/mL
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken BALP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken BALP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken BALP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken BALP in the samples is then determined by comparing the OD of the samples to the standard curve.
Chicken BALP(Bone-specific Alkaline Phosphatase) ELISA Kit

Chicken BALP(Bone-specific Alkaline Phosphatase) ELISA Kit

$320.00
$320.00