Chicken COL10(Collagen Type X) ELISA Kit

Item number: ELK9436
Availability: In Stock
Product Type: Precoated
$320.00
$320.00
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Size: 48T
Subtotal: $320.00
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Chicken COL10(Collagen Type X) ELISA Kit

Chicken COL10(Collagen Type X) ELISA Kit

$320.00

Chicken COL10(Collagen Type X) ELISA Kit

$320.00
Size: 48T
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken COL10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken COL10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken COL10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken COL10 in the samples is then determined by comparing the OD of the samples to the standard curve.
Uniprot id
Q2LK95
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Research area
Metabolic pathway;Developmental science;Nutrition metabolism;Bone metabolism;
Assay length
3.5h
Sample type
Serum, plasma, tissue homogenates and other biological fluids
Detection range
0.32-20 ng/mL
Standard
20 ng/mL
Sensitivity
0.113 ng/mL
Assay type
Sandwich
Reactivity
Chicken

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