This CytoSoft Imaging 96-well product has a defined elastic modulus (see certificate of analysis) with a glass bottom for high resolution imaging The thickness of the silicone gel is uniform with a ~0.03 mm thick layer of silicone in each well. The silicone gels are activated and ready to bind to a purified ECM. The rigidity of the substrate to which cells adhere can have a profound effect on cell morphology and gene expression. The CytoSoft Imaging plate has a glass bottom for high-resolution imaging where low autofluorescence and exceptional optical clarity are required. The 96-well format is optimal for high-throughput cell studies and screening. On the bottom of each well, there is a thin layer of specially formulated biocompatible silicone, whose elastic modulus (rigidity) is carefully measured and certified.