EasyStep Mouse E2(Estradiol) ELISA Kit

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse E2 antigen, and the Mouse E2 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Mouse E2 to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Mouse E2 in the samples is then determined by comparing the OD of the samples to the standard curve.

around 3 working days

L 16cm x W 15cm x H 9cm

Endocrinology;Reproductive science;Hormone metabolism;

1.5h

Serum

25-1600 pg/mL

1600 pg/mL

25 pg/mL

Competitive Inhibition

Mouse