This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with FK506. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to FK506. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of FK506 in the samples is then determined by comparing the OD of the samples to the standard curve.

around 3 working days

L 16cm x W 15cm x H 9cm

Immunology; Biochemicals

2h

Serum, plasma, cell lysates, cell culture supernates and other biological fluids

0.16-10 ng/mL

10 ng/mL

0.051 ng/mL

Competitive Inhibition

General