Goat IgM(Immunoglobulin M) ELISA Kit

Item number: ELK9969
Availability: In Stock
Product Type: Precoated
$588.00
$588.00
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Size: 96T
Subtotal: $588.00
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Goat IgM(Immunoglobulin M) ELISA Kit

Goat IgM(Immunoglobulin M) ELISA Kit

$588.00

Goat IgM(Immunoglobulin M) ELISA Kit

$588.00
Size: 96T
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Goat IgM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Goat IgM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Goat IgM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Goat IgM in the samples is then determined by comparing the OD of the samples to the standard curve.
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Research area
Infection immunity;Immune molecule;Hematology;
Assay length
3.5h
Sample type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection range
31.25-2000 ng/mL
Standard
2000 ng/mL
Sensitivity
14.3 ng/mL
Assay type
Sandwich
Reactivity
Goat

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