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Guinea pig NF-κB p105(Nuclear factor NF-kappa-B p105 subunit) ELISA Kit
Guinea pig NF-κB p105(Nuclear factor NF-kappa-B p105 subunit) ELISA Kit

Guinea pig NF-κB p105(Nuclear factor NF-kappa-B p105 subunit) ELISA Kit

Guinea pig NF-κB p105(Nuclear factor NF-kappa-B p105 subunit) ELISA Kit

Vendor: ELK Biotechnology

Type: Precoated

SKU: ELK9033

$320.00
$320.00
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Available in stock (100)

Size: 48T
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Product Overview

Assay length
3.5h
Assay type
Sandwich
Detection range
0.32-20 ng/mL
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Reactivity
Guinea pig
Research area
Signal transduction;
Sample type
tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
0.116 ng/mL
Standard
20 ng/mL
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Guinea pig NF-κBp105. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Guinea pig NF-κBp105. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Guinea pig NF-κBp105, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Guinea pig NF-κBp105 in the samples is then determined by comparing the OD of the samples to the standard curve.
Guinea pig NF-κB p105(Nuclear factor NF-kappa-B p105 subunit) ELISA Kit

Guinea pig NF-κB p105(Nuclear factor NF-kappa-B p105 subunit) ELISA Kit

$320.00
$320.00