Hamster TNF-a(Tumor Necrosis Factor-alpha) ELISA Kit

Item number: ELK0928
Availability: In Stock
Product Type: Precoated
$376.00
$376.00
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Size: 96T
Subtotal: $376.00
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Hamster TNF-a(Tumor Necrosis Factor-alpha) ELISA Kit

Hamster TNF-a(Tumor Necrosis Factor-alpha) ELISA Kit

$376.00

Hamster TNF-a(Tumor Necrosis Factor-alpha) ELISA Kit

$376.00
Size: 96T
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hamster TNF-a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hamster TNF-a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hamster TNF-a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hamster TNF-a in the samples is then determined by comparing the OD of the samples to the standard curve.
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Research area
Cytokine;Tumor immunity;Infection immunity;
Assay length
3.5h
Sample type
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection range
3.13-200 pg/mL
Standard
200 pg/mL
Sensitivity
1.09 pg/mL
Assay type
Sandwich
Reactivity
Hamster

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