Horse APLN(Apelin) ELISA Kit

Item number: ELK9472
Availability: In Stock
Product Type: Precoated
$320.00
$320.00
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Size: 48T
Subtotal: $320.00
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Horse APLN(Apelin) ELISA Kit

Horse APLN(Apelin) ELISA Kit

$320.00

Horse APLN(Apelin) ELISA Kit

$320.00
Size: 48T
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse APLN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse APLN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse APLN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse APLN in the samples is then determined by comparing the OD of the samples to the standard curve.
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Research area
Endocrinology;
Assay length
3.5h
Sample type
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection range
125-8000 pg/mL
Standard
8000 pg/mL
Sensitivity
46 pg/mL
Assay type
Sandwich
Reactivity
Horse

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