Horse HMGB-2(HighMobility Group Protein B2) ELISA Kit

Item number: ELK9976
Availability: In Stock
Product Type: Precoated
$588.00
$588.00
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Size: 96T
Subtotal: $588.00
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Horse HMGB-2(HighMobility Group Protein B2) ELISA Kit

Horse HMGB-2(HighMobility Group Protein B2) ELISA Kit

$588.00

Horse HMGB-2(HighMobility Group Protein B2) ELISA Kit

$588.00
Size: 96T
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse HMGB-2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse HMGB-2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse HMGB-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse HMGB-2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Research area
Infection immunity;
Assay length
3.5h
Sample type
Serum, plasma and other biological fluids
Detection range
0.16-10 ng/mL
Standard
10 ng/mL
Sensitivity
0.062 ng/mL
Assay type
Sandwich
Reactivity
Horse

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