Horse NF-κBp105(Nuclear factor NF-kappa-B p105 subunit) ELISA Kit

Item number: ELK9972
Availability: In Stock
Product Type: Precoated
$458.00
$458.00
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Size: 96T
Subtotal: $458.00
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Horse NF-κBp105(Nuclear factor NF-kappa-B p105 subunit) ELISA Kit

Horse NF-κBp105(Nuclear factor NF-kappa-B p105 subunit) ELISA Kit

$458.00

Horse NF-κBp105(Nuclear factor NF-kappa-B p105 subunit) ELISA Kit

$458.00
Size: 96T
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse NFKB-p105. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse NFKB-p105. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse NFKB-p105, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse NFKB-p105 in the samples is then determined by comparing the OD of the samples to the standard curve.
Uniprot id
F6TME6
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Research area
Signal transduction;
Assay length
3.5h
Sample type
tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection range
0.16-10 ng/mL
Standard
10 ng/mL
Sensitivity
0.059 ng/mL
Assay type
Sandwich
Reactivity
Horse

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