Horse NFkB2(Nuclear Factor Kappa B2) ELISA Kit

Item number: ELK9973
Availability: In Stock
Product Type: Precoated
$458.00
$458.00
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Size: 96T
Subtotal: $458.00
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Horse NFkB2(Nuclear Factor Kappa B2) ELISA Kit

Horse NFkB2(Nuclear Factor Kappa B2) ELISA Kit

$458.00

Horse NFkB2(Nuclear Factor Kappa B2) ELISA Kit

$458.00
Size: 96T
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse NFkB2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse NFkB2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse NFkB2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse NFkB2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Research area
Signal transduction;
Assay length
3.5h
Sample type
Tissue homogenates and other biological fluids.
Detection range
0.32-20 ng/mL
Standard
20 ng/mL
Sensitivity
0.131 ng/mL
Assay type
Sandwich
Reactivity
Horse

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