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Human 15-HETE(15-Hydroxyeicosatetraenoic Acid) ELISA Kit
Human 15-HETE(15-Hydroxyeicosatetraenoic Acid) ELISA Kit

Human 15-HETE(15-Hydroxyeicosatetraenoic Acid) ELISA Kit

Human 15-HETE(15-Hydroxyeicosatetraenoic Acid) ELISA Kit

Vendor: ELK Biotechnology

Type: Precoated

SKU: ELK8351

$320.00
$320.00
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Available in stock (100)

Size: 48T
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Product Overview

Assay length
2h
Assay type
Competitive Inhibition
Detection range
0.13-8 ng/mL
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Reactivity
Human
Research area
Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology;
Sample type
serum, plasma, cell lysates, cell culture supernates and other biological fluids
Sensitivity
0.1 ng/mL
Standard
8 ng/mL
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 15-HETE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 15-HETE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 15-HETE in the samples is then determined by comparing the OD of the samples to the standard curve.
Human 15-HETE(15-Hydroxyeicosatetraenoic Acid) ELISA Kit

Human 15-HETE(15-Hydroxyeicosatetraenoic Acid) ELISA Kit

$320.00
$320.00