Human a2M(Alpha-2-Macroglobulin) ELISA Kit

Item number: ELK10189
Availability: In Stock
Product Type: Precoated
$265.00
$265.00
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Size: 48T
Subtotal: $265.00
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Human a2M(Alpha-2-Macroglobulin) ELISA Kit

Human a2M(Alpha-2-Macroglobulin) ELISA Kit

$265.00

Human a2M(Alpha-2-Macroglobulin) ELISA Kit

$265.00
Size: 48T
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human a2M. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human a2M. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human a2M, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human a2M in the samples is then determined by comparing the OD of the samples to the standard curve.
Uniprot id
P01023
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Research area
Tumor immunity;Infection immunity;Hematology;
Assay length
3.5h
Sample type
serum, plasma, urine and other biological fluids
Detection range
1.57-100 µg/mL
Standard
100 µg/mL
Sensitivity
0.48 µg/mL
Assay type
Sandwich
Reactivity
Human

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