Human ACTN3(Actinin Alpha 3) ELISA Kit

Item number: ELK5224
Availability: In Stock
Product Type: Precoated
$265.00
$265.00
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Size: 48T
Subtotal: $265.00
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Human ACTN3(Actinin Alpha 3) ELISA Kit

Human ACTN3(Actinin Alpha 3) ELISA Kit

$265.00

Human ACTN3(Actinin Alpha 3) ELISA Kit

$265.00
Size: 48T
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ACTN3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ACTN3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ACTN3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ACTN3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Uniprot id
Q08043
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Research area
Metabolic pathway;Developmental science;Bone metabolism;
Assay length
3.5h
Sample type
Tissue homogenates, cell lysates and other biological fluids.
Detection range
1.57-100 ng/mL
Standard
100 ng/mL
Sensitivity
0.64 ng/mL
Assay type
Sandwich
Reactivity
Human

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