Human ANA(anti-nuclear Antibody) ELISA Kit

Item number: ELK9201
Availability: In Stock
Product Type: Precoated
$320.00
$320.00
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Size: 48T
Subtotal: $320.00
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Human ANA(anti-nuclear Antibody) ELISA Kit

Human ANA(anti-nuclear Antibody) ELISA Kit

$320.00

Human ANA(anti-nuclear Antibody) ELISA Kit

$320.00
Size: 48T
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ANA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ANA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ANA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ANA in the samples is then determined by comparing the OD of the samples to the standard curve.
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Research area
Biochemical research
Assay length
3.5h
Sample type
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection range
1.57-100 ng/mL
Standard
100 ng/mL
Sensitivity
0.56 ng/mL
Assay type
Sandwich
Reactivity
Human

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