Human Anti-tTG-IgA (Anti-Tissue Transglutaminase IgA) ELISA Kit

Item number: ELK0752
Availability: In Stock
Product Type: Precoated
$458.00
$458.00
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Size: 96T
Subtotal: $458.00
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Human Anti-tTG-IgA (Anti-Tissue Transglutaminase IgA) ELISA Kit

Human Anti-tTG-IgA (Anti-Tissue Transglutaminase IgA) ELISA Kit

$458.00

Human Anti-tTG-IgA (Anti-Tissue Transglutaminase IgA) ELISA Kit

$458.00
Size: 96T
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-tTG-IgA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-tTG-IgA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-tTG-IgA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-tTG-IgA in the samples is then determined by comparing the OD of the samples to the standard curve.
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Research area
Enzyme & Kinase;Endocrinology;Neuro science;Autoimmunity;
Assay length
3.5h
Sample type
Serum, plasma, tissue homogenates and other biological fluids.
Detection range
4.69-300 IU/mL
Standard
300 IU/mL
Sensitivity
1.45 IU/mL
Assay type
Sandwich
Reactivity
Human

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