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Human ATG12(Autophagy Related Protein 12) ELISA Kit
Human ATG12(Autophagy Related Protein 12) ELISA Kit

Human ATG12(Autophagy Related Protein 12) ELISA Kit

Human ATG12(Autophagy Related Protein 12) ELISA Kit

Vendor: ELK Biotechnology

Type: Precoated

SKU: ELK7517

$320.00
$320.00
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Manual
SDS

Available in stock (100)

Size: 48T
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Product Overview

Assay length
3.5h
Assay type
Sandwich
Detection range
0.16-10 ng/mL
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Reactivity
Human
Research area
Metabolic pathway;
Sample type
Tissue homogenates, cell lysates and other biological fluids
Sensitivity
0.056 ng/mL
Standard
10 ng/mL
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ATG12. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ATG12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ATG12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ATG12 in the samples is then determined by comparing the OD of the samples to the standard curve.
Uniprot id
O94817
Human ATG12(Autophagy Related Protein 12) ELISA Kit

Human ATG12(Autophagy Related Protein 12) ELISA Kit

$320.00
$320.00