The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CA72-4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CA72-4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CA72-4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CA72-4 in the samples is then determined by comparing the OD of the samples to the standard curve.

around 3 working days

L 16cm x W 15cm x H 9cm

Tumor immunity;Infection immunity;

3.5h

serum, plasma and other biological fluids

0.63-40 U/mL

40 U/mL

0.3 U/mL

Sandwich

Human