The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GPX7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GPX7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GPX7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GPX7 in the samples is then determined by comparing the OD of the samples to the standard curve.

Q96SL4

around 3 working days

L 16cm x W 15cm x H 9cm

Enzyme & Kinase;Metabolic pathway;

3.5h

Serum, plasma, tissue homogenates, cell lysates and other biological fluids

0.16-10 ng/mL

10 ng/mL

0.071 ng/mL

Sandwich

Human