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Human HSPBP1(Heat Shock 70kDa Binding Protein 1) ELISA Kit

Human HSPBP1(Heat Shock 70kDa Binding Protein 1) ELISA Kit

Regular price $320.00
Sale price $320.00 Regular price
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HSPBP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HSPBP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HSPBP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HSPBP1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Uniprot id
Q9NZL4
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Research area
Infection immunity;
Assay length
3.5h
Sample type
Serum, plasma and other biological fluids
Detection range
0.32-20 ng/mL
Standard
20 ng/mL
Sensitivity
0.121 ng/mL
Assay type
Sandwich
Reactivity
Human