Human IFA(Intrinsic Factor Antibody) ELISA Kit

Item number: ELK9327
Availability: In Stock
Product Type: Precoated
$320.00
$320.00
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Size: 48T
Subtotal: $320.00
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Human IFA(Intrinsic Factor Antibody) ELISA Kit

Human IFA(Intrinsic Factor Antibody) ELISA Kit

$320.00

Human IFA(Intrinsic Factor Antibody) ELISA Kit

$320.00
Size: 48T
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IFA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IFA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IFA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IFA in the samples is then determined by comparing the OD of the samples to the standard curve.
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Research area
Signal transduction; Metabolism
Assay length
3.5h
Sample type
Serum, plasma, tissue homogenates and other biological fluids.
Detection range
78.13-5000 pg/mL
Standard
5000 pg/mL
Sensitivity
30 pg/mL
Assay type
Sandwich
Reactivity
Human

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