Human IgA(Immunoglobulin A) ELISA Kit

Item number: ELK1255
Availability: In Stock
Product Type: Precoated
$376.00
$376.00
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Size: 96T
Subtotal: $376.00
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Human IgA(Immunoglobulin A) ELISA Kit

Human IgA(Immunoglobulin A) ELISA Kit

$376.00

Human IgA(Immunoglobulin A) ELISA Kit

$376.00
Size: 96T
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IgA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IgA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IgA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IgA in the samples is then determined by comparing the OD of the samples to the standard curve.
Uniprot id
P01876
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Research area
Infection immunity;Immune molecule;Hematology;Pulmonology;
Assay length
3.5h
Sample type
serum, plasma, saliva and other biological fluids
Detection range
1.57-100 µg/mL
Standard
100 µg/mL
Sensitivity
0.51 µg/mL
Assay type
Sandwich
Reactivity
Human

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