Human IL18(Interleukin 18) ELISA Kit

Item number: ELK1245
Availability: In Stock
Product Type: Precoated
$376.00
$376.00
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Size: 96T
Subtotal: $376.00
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Human IL18(Interleukin 18) ELISA Kit

Human IL18(Interleukin 18) ELISA Kit

$376.00

Human IL18(Interleukin 18) ELISA Kit

$376.00
Size: 96T
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL18. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL18. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL18, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL18 in the samples is then determined by comparing the OD of the samples to the standard curve.
Uniprot id
Q14116
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Research area
Cytokine;Infection immunity;
Assay length
3.5h
Sample type
serum, plasma, urine, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection range
125-8000 pg/mL
Standard
8000 pg/mL
Sensitivity
37.2 pg/mL
Assay type
Sandwich
Reactivity
Human

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