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Human ISG15(Interferon Stimulated Gene 15) ELISA Kit
Human ISG15(Interferon Stimulated Gene 15) ELISA Kit

Human ISG15(Interferon Stimulated Gene 15) ELISA Kit

Human ISG15(Interferon Stimulated Gene 15) ELISA Kit

Vendor: ELK Biotechnology

Type: Precoated

SKU: ELK6581

$320.00
$320.00
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Available in stock (100)

Size: 48T
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Product Overview

Assay length
3.5h
Assay type
Sandwich
Detection range
31.25-2000 pg/mL
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Reactivity
Human
Research area
Cytokine;
Sample type
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
11.9 pg/mL
Standard
2000 pg/mL
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ISG15. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ISG15. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ISG15, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ISG15 in the samples is then determined by comparing the OD of the samples to the standard curve.
Human ISG15(Interferon Stimulated Gene 15) ELISA Kit

Human ISG15(Interferon Stimulated Gene 15) ELISA Kit

$320.00
$320.00