New

Large collection of high-quality Antibodies!
Human MDM2(E3 ubiquitin-protein ligase Mdm2) ELISA Kit
Human MDM2(E3 ubiquitin-protein ligase Mdm2) ELISA Kit

Human MDM2(E3 ubiquitin-protein ligase Mdm2) ELISA Kit

Human MDM2(E3 ubiquitin-protein ligase Mdm2) ELISA Kit

Vendor: ELK Biotechnology

Type: Precoated

SKU: ELK8761

$320.00
$320.00
Shipping calculated at checkout.

Documents

Manual
SDS

Available in stock (100)

Size: 48T
View UniProt Entry
×

Request a Quote

Limited time offer

Get $200 off when you spend $2,500 or more!

Product Overview

Assay length
3.5h
Assay type
Sandwich
Detection range
0.16-10 ng/mL
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Reactivity
Human
Research area
Apoptosis
Sample type
serum, plasma, other biological fluids
Sensitivity
0.07 ng/mL
Standard
10 ng/mL
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Mdm2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Mdm2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Mdm2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Mdm2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Uniprot id
Q00987
Human MDM2(E3 ubiquitin-protein ligase Mdm2) ELISA Kit

Human MDM2(E3 ubiquitin-protein ligase Mdm2) ELISA Kit

$320.00
$320.00