Human PCNA(Proliferating Cell Nuclear Antigen) ELISA Kit

Item number: ELK5141
Availability: In Stock
Product Type: Precoated
$265.00
$265.00
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Size: 48T
Subtotal: $265.00
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Human PCNA(Proliferating Cell Nuclear Antigen) ELISA Kit

Human PCNA(Proliferating Cell Nuclear Antigen) ELISA Kit

$265.00

Human PCNA(Proliferating Cell Nuclear Antigen) ELISA Kit

$265.00
Size: 48T
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PCNA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PCNA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PCNA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PCNA in the samples is then determined by comparing the OD of the samples to the standard curve.
Uniprot id
P12004
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Research area
Tumor immunity;Developmental science;
Assay length
3.5h
Sample type
tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection range
0.16-10 ng/mL
Standard
10 ng/mL
Sensitivity
0.057 ng/mL
Assay type
Sandwich
Reactivity
Human

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