This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human PIIICP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PIIICP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PIIICP in the samples is then determined by comparing the OD of the samples to the standard curve.

around 3 working days

L 16cm x W 15cm x H 9cm

Metabolic pathway;Bone metabolism;

2h

serum, plasma, cell lysates, cell culture supernates and other biological fluids

3.13-200 ng/mL

200 ng/mL

0.96 ng/mL

Competitive Inhibition

Human