Human RANKL(Receptor Activator of Nuclear factor-kB Ligand) ELISA Kit

Item number: ELK9869
Availability: In Stock
Product Type: Precoated
$320.00
$320.00
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Size: 48T
Subtotal: $320.00
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Human RANKL(Receptor Activator of Nuclear factor-kB Ligand) ELISA Kit

Human RANKL(Receptor Activator of Nuclear factor-kB Ligand) ELISA Kit

$320.00

Human RANKL(Receptor Activator of Nuclear factor-kB Ligand) ELISA Kit

$320.00
Size: 48T
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human RANKL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human RANKL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human RANKL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human RANKL in the samples is then determined by comparing the OD of the samples to the standard curve.
Uniprot id
O14788
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Research area
Signal transduction;Metabolic pathway;Apoptosis;Tumor immunity;Bone metabolism;
Assay length
3.5h
Sample type
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection range
78.13-5000 pg/mL
Standard
5000 pg/mL
Sensitivity
23.6 pg/mL
Assay type
Sandwich
Reactivity
Human

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